BioChaga

BioChaga project 

Poze site

Titlu/Title

Metoda de crestere a sintezei compusilor bioactivi in miceliul ciupercii fitoparazite Inonotus obliquus, prin fermentatie submersa

Method for enhanced synthesis of bioactive compounds in mycelium of phytoparasitic mushroom Inonotus obliquus, by submerged fermentation

Cod proiect/Project code: 

Proiect Experimental Demonstrativ
(Ctr. PED 171 / 2017)

Acronim: BIOChaga

Proiect (luni)/Project Duration (month): 16 luni/months (sept. 2017 – dec. 2018)

Buget proiect/Total Funding (LEI): 475.000 lei

Echipa proiectului/Project team:

Coordonator/Project manager: IFIN-HH (Dir. Dr. Mihaela ENE)

Partener/Partner 1: USAMVB (Resp. Dr. Emanuel VAMANU)

Proiect/Project: 

Multe specii de ciuperci au demonstrat proprietati medicinale si au fost dintotdeauna folosite in medicina traditionala ca antitumorale, antibiotice, antiparazitice, antivirale, antiinflamatorii, imunostimulatoare si antidiabetice. Compusi bine cunoscuti, precum cordicepina din Cordyceps militaris sau fractiile polizaharid-peptidice din miceliul de Coryolus versicolor (patentate sub denumirea de PSP si PSK) sunt deja inclusi in preparate farmaceutice sau nutraceutice consacrate si prescrise ca adjuvanti in diferite forme de cancer. Antioxidantii formeaza o clasa importanta de compusi extrasi din ciuperci, fie din corpul de fructificatie, fie din miceliul acestora.

Proiectul isi propune optimizarea si validarea in laborator a unei metode de crestere a continutului sau activitatii antioxidante a miceliului de Inonotus obliquus (Chaga), in urma stimularii prin iradiere gamma. Fiind o specie rara in natura si cu crestere lenta, vom prefera metoda de fermentatie submersa a miceliului. Rezultatele preliminare arata ca potentialul antioxidant creste intr-o maniere dependenta de doza, la o strategie de iradiere secventiala. Optimizarea vizeaza gasirea unor ingrediente si conditii profitabile economic (temperatura de cultivare, numarul de stimulari si doza, varsta de recoltare) astfel incat metoda de cultivare si extractie sa fie aplicabila la scara industriala. Validarea metodei va fi completata de formularea unei proceduri standard de operare ce acopera atat producerea miceliului pe loturi cat si testarea calitatii extractului. Odata patentata, metoda va fi accesibila industriei farmaceutice si disponibila pentru obtinere si caracterizare de subfractii chimice de interes medical.

Obiectivul proiectului

Dezvoltarea unei tehnologii (demonstrator experimental) de stimulare a sintezei de antioxidanti in miceliul ciupercii Inonotus obliquus, cultivat submers, prin tratament cu radiatii gamma.

A lot of mushrooms demonstrate medicinal properties and have been traditionally used for their antitumor, antibiotic, antiparasitic, antiviral, anti-inflammatory activities, immune response stimulating effects, antidiabetic properties and blood lipid lowering effects. Famous bioactive compounds like cordycepin from Cordyceps militaris and Polysaccharide Peptide from Coryolus versicolor mycelium (patented fractions: PSP and PSK) are already included in pharmaceutical products or nutraceutics and are prescribed as adjuvants in the treatment of different forms of cancer. Antioxidants are one major class of bioactive compounds extracted from mushrooms, either fruit body or mycelium.

The project is proposing optimization and in-lab validation of a method to increase the antioxidant content or activity of the mycelium of Inonotus obliquus (Chaga), following stimulation with gamma irradiation. Because its slow growing and scarcity in nature, submerged fermentation of mycelium is the preferred cultivation method for this mushroom. Preliminary results shows that the antioxidant potential is elevated in a dose dependent manner, in a sequential stimulation strategy. The project is oriented to economically profitable ingredients and conditions (cultivation temperature, stimulation events and doses, age of harvesting) for the industrial scale cultivation and active compounds extraction. A standard operating procedure covering the production batch system and the quality testing of the extract will be issued to complete the validation. Once patented, the method will be available for pharmaceutical manufacturers as well as for further purification and advanced subfraction characterization studies.

Project objective

To develop a gamma irradiation-based technology (experimental demonstrator) of enhanced synthesis of antioxidants in the mycelium of Inonotus obliquus mushroom, in submerged fermentation.

 

Rezultatele etapei I (2017)

  1. A fost izolata si identificata prin PCR cu primeri specifici tulpina de Inonotus obliquus.
  2. A fost partial optimizata compozitia unui mediu de cultura lichid, pentru parametrii:
  • biomasa uscata de miceliu
  • con?inutului total de fenoli
  • con?inutului total de flavonoide
  • activitatea de inhibare a radicalului DPPH

Experimentele s-au desfasurat in vase cu agitare, variind sursa de C si/sau N, finalizandu-se astfel etapa de screening (identificare a componentilor esentiali); urmeaza etapele de determinare a maximului de productivitate (prin modularea cantitatii componentelor) si experimetele de stimulare a producerii de antioxidanti prin iradiere.